Rapid method for quantifying prenylated proteins developed

Friday, September 16, 2016 - 11:30am

The work of former students Josh Ochocki (now at Pfizer) and Charuta Palsuledesai (now at the University of new Mexico) performed in collaboraton with the Arriaga, Li and Wattenberg groups at the Univerrsity of Minnesota recently appeared in an article published in ACS Chemical Biology.  In that paper, alkyne-containing isoprenoid analogues were used to visualize and quantify levels of prenylated proteins.  In both cases, detection was accomplished by click reaction with an azide-containing TAMRA-based probe. Visualization was done via confocal microscopy while quantification was performed via flow cytometry.  Using a cell culture-based model for aging (reduced levels of autophagy), they were able to shown that higher levels of prenylated proteins were found in the "aging" cells.  These results provide interesting new directions to study age-related disorders including Alzheimer's disease.