2024 | |
| 208 Upstream proteolysis by Ste24 does not require a C-terminal methyl ester as revealed by 33-residue a-factor precursor peptides synthesized using epimerization-free methods Bader, T. K.; Brown, S. M.; Hrycyna, C. A.; Distefano, M. D. Upstream proteolysis by Ste24 does not require a C-terminal methyl ester as revealed by 33-residue a-factor precursor peptides synthesized using epimerization-free methods, Int. J. Pep. Res. Ther. 2004, in press. DOI |
| 207 An alkyne-containing isoprenoid analogue based on a farnesyl diphosphate scaffold is a biologically functional universal probe for proteomic analysis Auger, S. A.; Pederson, J. S.; Maity, S.; Sprague-Getsy, A. M.; Lorimer, E. L.; Koehn, O. J.; Krauklis, S. A.; Berns, B.; Murphy, K. M.; Hussain, J.; Thao, P.; Bernhagen, K. B.; Justyna, K.; Sundaresan, A. P.; McKim, D. B.; Williams, C. L.; Hougland, J. L.; Li, L. Distefano, M. D. An alkyne-containing isoprenoid analogue based on a farnesyl diphosphate scaffold is a biologically functional universal probe for proteomic analysis, Biochemistry, 2024, in press, PMC in progress. |
| |
| 205 Library Screening, In Vivo Confirmation, and Structural and Bioinformatic Analysis of Pentapeptide Sequences as Substrates for Protein Farnesyltransferase Schey, G. L.; Hildebrandt, E. R.; Wang, Y.; Diwan, S.; Passetti, H. A.; Potts, G. A.; Sprague-Getsy, A. M.; Leoni, E. R.; Kuebler, T. S.; Sham, Y. Y.; Hougland, J. L.; Beese, L. S.; Schmidt, W. K.; Distefano, M. D. Library Screening, In Vivo Confirmation, and Structural and Bioinformatic Analysis of Pentapeptide Sequences as Substrates for Protein Farnesyltransferase, Int. J. Mol. Sci. 2024, 25, 5324, PMC11121372. DOI |
| 204 Macrophage cytoskeletal remodeling in response to the chemoattractant C5a is dependent on geranylgeranyl pyrophosphate Kauklis, S. A.; Hussainb, J.; Murphy, K. M.; Dray, E. L.; Justyna, K.; Distefano, M. D.; Steelmana, A. J.; McKim, D. B. Macrophage cytoskeletal remodeling in response to the chemoattractant C5a is dependent on geranylgeranyl pyrophosphate, Am. J. Physiol. Endocrinol. Metab. 2024, 327, E55-E68, PMC11390116. DOI |
| 203 Targeted Drug Delivery by MMAE Farnesyl-Bioconjugated Multivalent Chemically Self-Assembled Nanorings Induces Potent Receptor-Dependent Immunogenic Cell Death Wang, Y.; Kilic, O.; Rozumalski, L.; Distefano, M. D.; Wagner. C. R. Targeted Drug Delivery by MMAE Farnesyl-Bioconjugated Multivalent Chemically Self-Assembled Nanorings Induces Potent Receptor-Dependent Immunogenic Cell Death, Bioconj. Chem., 2024, 35, 582-592, PMC11633779. |
| 202 Broadening the utility of farnesyltransferase-catalyzed protein labeling using norbornene-tetrazine click chemistry Auger, S. A.; Venkatachalapathy, S.; Suazo, K. F. G.; Wang, Y.; Sarkis, A. W.; Bernhagen, K. B.; Justyna, K.; Schaefer, J. V.; Wollack, J. W.; Plückthun, A.; Li, L.; Distefano, M. D. Broadening the utility of farnesyltransferase-catalyzed protein labeling using norbornene-tetrazine click chemistry, Bioconj. Chem., 2024, 35, 922-933, PMC11619650. |
| 201 Improved synthesis and application of an alkyne-functionalized isoprenoid analogue to study the prenylomes of motor neurons, astrocytes and their stem cell progenitors Suazo, K. F.; Mishra, V.; Maity, S. A.; Auger, S. A.; Justyna, K.; Petre, A. M.; Ottoboni, L.; Ongaro, J.; Corti, S. C.; Lotti, F.; Przedborski, S.; Distefano, M. D. Improved synthesis and application of an alkyne-functionalized isoprenoid analogue to study the prenylomes of motor neurons, astrocytes and their stem cell progenitors, Bioorg. Chem., 2024, 147, 107365, PMC in progress. |
| 200 Multivalent Fluorinated Nanorings for On-Cell 19F NMR, Li, J.; Wang, Y.; Distefano, M. D.; Wagner, C. R.; Pomerantz, W. C. K. Multivalent Fluorinated Nanorings for On-Cell 19F NMR, Biomacromolecules 2024 25, 1330-1339, PMC11375447. |
2023 | |
| 199 An orthogonal enzyme-substrate design strategy for discovery of human protein palmitoyltransferase substrates Puthenveetil, R.; Auger, S.; Gómez-Navarro, N.; Rana, M.; Das, R.; Healy, L.; Suazo, K.; Shi, Z.-D.; Swenson, R.; Distefano, M. D.; Banerjee, A. An orthogonal enzyme-substrate design strategy for discovery of human protein palmitoyltransferase substrates, J. Am. Chem. Soc., 2023, 145, 22287-22292, PMC10591334. |
| 198 Synthesis, enzymatic peptide incorporation and applications of diazirine-containing isoprenoid diphosphate analogues Justyna, K.; Das, R.; Lorimer, E. L.; Hu, J.; Pedersen, J. S.; Sprague-Getsy, A. M.; Schey, G. L.; Sieburg, M. A.; Koehn, O. J.; Wang, Y.-C.; Chen, Y.-X.; Hougland, J. L.; Williams, C. L.; Distefano, M. D. Synthesis, enzymatic peptide incorporation and applications of diazirine-containing isoprenoid diphosphate analogues, Org. Lett, 2023, 25, 6767-6772, PMC10755972. |
| 197 Thinking outside the CaaX-box: an unusual reversible prenylation on ALDH9A1 Suazo, K. F.; Bělíček, J.; Schey, G. L.; Auger, S. A.; Li, L.; Błażewska, K. M.; Kopečný, D.; Distefano M. D. Thinking outside the CaaX-box: an unusual reversible prenylation on ALDH9A1, RSC Chem. Biol., 2023, 4, 913-925, PMC10619140. |
| 196 Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl-derivatized Sepharose 4B Lee, K.-H.; Distefano, M. D.; Seelig, B. Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl-derivatized Sepharose 4B, Res. Chem. 2023, 6, 101044. |
| 195 Functional Screening Reveals Toxoplasma Prenylated Proteins Required for Endocytic Trafficking and Rhoptry Protein Sorting Wang, Q.; Sun, M.; Tang, T.; Lai, D.-H.; Liu, J.; Maity, M.; He, K.; Wu, X.-T.; Yang, J.; Li, Y.-B.; Tang, X.-Y.; Ding, H.-Y.; Hide, G.; Distefano, M. D.; Lun, Z.-R.; Zhu, X.-Q.; Long, S. Functional Screening Reveals Toxoplasma Prenylated Proteins Required for Endocytic Trafficking and Rhoptry Protein Sorting, mBio, 2023, 14, e01309. |
| 194 Prenylcysteine oxidase 1 like protein is required for neutrophil bactericidal activitiesPetenkova, A.; Auger, S. A.; Lamb, J.; Quellier, D.; Carter, C.; To, O. T.; Milosevic, J.; Barghout, R.; Kugadas, A.; Lu, X.; Geddes-McAlister, J.; Fichorova, R.; Sykes, D. B.; Distefano, M. D.; Gadjeva, M. Prenylcysteine oxidase 1 like protein is required for neutrophil bactericidal activities. Nat. Comm., 2023, 14, 2761. |
| 193 GTPase splice variants RAC1 and RAC1B display isoform-specific differences in localization, prenylation, and interaction with the chaperone protein SmgGDSKoehn, O. J.; Lorimer, E.; Unger, B.; Harris, R. M.; Das, A. S.; Suazo, K. F.; Auger, S. A.; Distefano, M. D.; Prokop, J. W.; Williams, C. L. GTPase splice variants RAC1 and RAC1B display isoform-specific differences in localization, prenylation, and interaction with the chaperone protein SmgGDS. J. Biol. Chem., 2023, 299, 104698. |
2022 | |
| 192 Engineering Biomimetic Trogocytosis with Farnesylated Chemically Self-Assembled NanoringsWang, Y.; Rozumalski, L.; Lichtenfels, C.; Petersberg, J. R.; Kilic, O.; Distefano, M. D.; Wagner, C. R. Engineering Biomimetic Trogocytosis with Farnesylated Chemically Self-Assembled Nanorings. Biomacromol., 2022, 23, 5018-5035, PMC9869669. |
| 191 Photoswitchable Isoprenoid Lipids Enable Optical Control of Peptide LipidationMorstein, J.; Bader, T.; Cardillo, A. L.; Schackmannb, J.; Ashoke, S.; Hougland, J. L.; Hrycyna, C. A.; Trauner, D. H.; Distefano, M. D. Photoswitchable Isoprenoid Lipids Enable Optical Control of Peptide Lipidation. ACS Chem. Biol., 2022, 17, 2945–2953. |
| 190 Enzymatic Construction of DARPin-Based Targeted Delivery Systems using Protein Farnesyltransferase and a Capture and Release StrategyZhang, Y.; Wang, Y.; Uslu, S.; Venkatachalapathy, S.; Rashidian, M.; Schaefer, J. V.; Plückthun, A.; Distefano, M. D. Enzymatic Construction of DARPin-Based Targeted Delivery Systems using Protein Farnesyltransferase and a Capture and Release Strategy. Int. J. Mol. Sci., 2022, 23, 11537. |
| 189 Structure-guided discovery of potent antifungals that prevent Ras signaling by inhibiting protein farnesyltransferaseWang, Y.; Xu, F.; Nichols, C.; Shi, Y.; Hellinga, H.; Alspaugh , J. A.; Distefano, M. D.; Beese, L. Structure-guided discovery of potent antifungals that prevent Ras signaling by inhibiting protein farnesyltransferase. J. Med. Chem., 2022, 65, 20. |
| 188 Mevalonate metabolites contribute to granulocyte chemotaxis and mortality in murine endotoxemiaHussain, J.; Ousley, C. G.; Krauklis, S.A.; Dray, E. L.; Drnevich, J.; Justyna, K.; Distefano, M. D.; McKim, D. B. Mevalonate metabolites contribute to granulocyte chemotaxis and mortality in murine endotoxemia. bioRxiv 2022.09.13.507840. |
| 187 In vivo prenylomic profiling in the brain of a transgenic mouse model of Alzheimer’s disease reveals increased prenylation of a key set of proteinsJeong, A.; Auger, S. A.; Maity, S.; Fredriksen, K.; Zhong, R.; Li, L.; and Distefano, M. D. In vivo prenylomic profiling in the brain of a transgenic mouse model of Alzheimer’s disease reveals increased prenylation of a key set of proteins. ACS Chem. Biol., 2022, 17, 2863–2876. |
| 186 Thinking outside the CaaX-box: an unusual reversible prenylation on ALDH9A1Suazo, K. F.; Schey, G. L.; Auger, S. A.; Li, L.; Distefano, M. D. Thinking outside the CaaX-box: an unusual reversible prenylation on ALDH9A1. bioRxiv 2022.04.01.486487. |
| 185 Manipulating Cell Fates with Protein ConjugatesWang, Y.; Wagner, C. R.; Distefano, M. D. Manipulating Cell Fates with Protein Conjugates. Bioconj. Chem., 2022, 33, 10. |
| 184 Combining Isoprenoid Probes with Antibody Markers for Mass Cytometric Analysis of Prenylation in Single CellsMaxwell, Zoe; Suazo, Kiall; Brown, Heather; Distefano, Mark; Arriaga, Edgar. Combining Isoprenoid Probes with Antibody Markers for Mass Cytometric Analysis of Prenylation in Single Cells. Anal. Chem., 2022, 94, 11521-11528. |
| 183 Photoresponsive Hydrogels for Studying Mechanotransduction of Cells, in MechanosensingPark, KY., Odde, D.J., Distefano, M.D. (2023). Photoresponsive Hydrogels for Studying Mechanotransduction of Cells. In: Zaidel-Bar, R. (eds) Mechanobiology. Methods in Molecular Biology, vol 2600. Humana, New York, NY. p 133-153. |
| 182 Directed cell migration toward softer environmentsIsomursu, A.; Park, K.-Y.; Hou, J.; Cheng, B.; Shamsan, G.; Fuller, B.; Kasim, J.; Mahmoodi, M. M.; Lu, T. J.; Genin, G. M.; Xu, F.; Lin, M.; Distefano, M. D.; Ivaska, J.; Odde, D. J. Directed cell migration toward softer environments. Nat. Mater., 2022, 21, 1081-1090. |
| 181 In vivo prenylomic profiling in the brain of a transgenic mouse model of Alzheimer’s disease reveals increased prenylation of a key set of proteinsAngela Jeong,* Shelby A. Auger,* Sanjay Maity, Ling Li, and Mark D. Distefano. In vivo prenylomic profiling in the brain of a transgenic mouse model of Alzheimer’s disease reveals increased prenylation of a key set of proteins. bioRxiv 2022.04.01.486487 *These authors contributed to the paper equally |
| 180 Macro-Chemical Biology: Engineering Biomimetic Trogocytosis with Farnesylated Chemically Self-Assembled NanoringsYiao Wang, Lakmal Rozumalski, Caitlin Lichtenfels, Jacob R. Petersberg, Ozgun Kilic, Mark D. Distefano, Carston R. Wagner. Macro-Chemical Biology: Engineering Biomimetic Trogocytosis with Farnesylated Chemically Self-Assembled Nanorings. bioRxiv 2022.03.01.482559 |
| 179 Negative durotaxis: cell movement toward softer environmentsAleksi Isomursu, Keun-Young Park, Jay Hou, Bo Cheng, Ghaidan Shamsan, Benjamin Fuller, Jesse Kasim, M. Mohsen Mahmoodi, Tian Jian Lu, Guy M. Genin, Feng Xu, Min Lin, Mark Distefano, Johanna Ivaska, David J. Odde. bioRxiv 2020.10.27.357178 |
2021 | |
| 178 MALDI analysis of peptide libraries expands the scope of substrates for farnesyltransferaseSchey, G.L.; Buttery P.H.; Hildebrandt, E.R.; Novak, S.X.; Schmidt, W.K.; Hougland, J.L.; Distefano, M.D. Int. J. Mol. Sci., 2021, 22, 12042. |
| 177 A Not-So-Ancient Grease History: Click Chemistry and Protein Lipid ModificationsSuazo, K.F.; Park, K.-Y; Distefano, M.D. A Not-So-Ancient Grease History: Click Chemistry and Protein Lipid Modifications. Chem. Rev., 2021, 121, 12, 7178–7248
|
| 176 Metabolic labeling with an alkyne probe reveals similarities and differences in the prenylomes of several brain-derived cell lines and primary cellsSuazo, K. F; Jeong, A.; Ahmadi, M.; Brown, C.; Qu, W.; Li, L.; Distefano, M. D. Metabolic labeling with an alkyne probe reveals similarities and differences in the prenylomes of several brain-derived cell lines and primary cells. Sci. Rep. 2021, 11, 4367. |
| 175 Two-Photon Uncaging of Bioactive Thiols in Live Cells at Wavelengths above 800 nmHammers, M.D.; Hodny, M.H.; Bader, T. K.; Mahmoodi, M.M.; Fang, S.; Fenton,A.D.; Nurie, K.; Trial, H.O.; Xu, F.; Healy, A.T.; Ball, Z.T.; Blank, D.A.; Distefano, M.D. Two-Photon Uncaging of Bioactive Thiols in Live Cells at Wavelengths above 800 nm, Org. Biomol. Chem., 2021, 19, 2213-2223. |
| 174 Neuronal Protein Farnesylation Regulates Hippocampal Synaptic Plasticity and Cognitive FunctionQu, W.; Suazo, K.F.; Liu, W.; Cheng, S.; Jeong, A.; Hottman, D.; Yuan, L.-L.; Distefano, M.D.; Li, L. Neuronal Protein Farnesylation Regulates Hippocampal Synaptic Plasticity and Cognitive Function, Mol. Neurobiol., 2021, 58, 1128–1144. |
| 173 Engineering Reversible Cell-Cell Interactions Using Enzymatically Lipidated Chemically Self-Assembled NanoringsWang, Y.; Kilic, O.; Csizmar, C.M.; Ashok, S.; Hougland, J.L.; Distefano, M.D.; Wagner, C. R. Engineering Reversible Cell-Cell Interactions Using Enzymatically Lipidated Chemically Self-Assembled Nanorings, Chem. Sci., 2021, 12, 331-340. |
Publications before 2021
2020
172 Kilic, O.; Matos de Souza, M.; Almotlak, A.; Wang, Y.; Siegfried, J.; Distefano, M. D.; Wagner, C. R. Anti-EGFR Fibronectin Bispecific Chemically Self-Assembling Nanorings (CSANs) Induce Potent T-cell Mediated Anti-Tumor Response and Downregulation of EGFR Signaling and PD-1/PD-L1 Expression J. Med. Chem., 2020, 63, 10235−10245. DOI
171. Mangubat-Medina, A. E.; Trial, H. O.; Vargas, R. D.; Setegne, M. T.; Bader, T.; Distefano, M. D.; Ball, Z. T. Red-shifted backbone N–H photocaging agents. Org. Biomol. Chem., 2020,18, 5110-5114. DOI
170. Brandt, A. C.; McNally, L.; Lorimer, E. L.; Unger, B.; Koehn, O. J.; Suazo, K. F.; Rein, L.; Szabo, A.; Tsaih, S.-W.; Distefano, M. D.; . Flister, M. J.; Rigo, F.; McNally, M. T.; Williams, C L. Splice switching an oncogenic ratio of SmgGDS isoforms as a strategy to diminish malignancy Proc. Nat. Acad. Sci. U.S.A. 2020, 117, 3627-3636, PMC7035473. DOI
169. Bader, T. K.; Xu, F.; Hodny, M. H.; Blank, D. A.; Distefano, M. D. A methoxy-substituted nitrodibenzofuran-based protecting group with an improved two-photon action cross-section for thiol protection in solid phase peptide synthesis J. Org. Chem. 2020, 85, 1614–1625, PMC7328522. DOI
2019
168. Zhang, Y.; Auger, S.; Schaefer, J. V.; Plückthun, A.; Distefano, M. D. (2019) Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransferase in Methods in Molecular Biology: Bioconjugation (Massa, S., Ed.), Springer, New York, p 207-219. DOI
167. Hsu, E-. T.; Vervacke, J. S.; Distefano, M. D.; Hrycyna, C. A. (2019) A quantitative FRET assay for the upstream cleavage activity of the integral membrane proteases human ZMPSTE24 and yeast Ste24
in Protein Lipidation: Methods and Protocols (Linder, M., Ed.), Springer, New York, p 279-293, https://link.springer.com/protocol/10.1007%2F978-1-4939-9532-5_21.
166. Ahmadi, M.; Suazo, K. F.; Distefano, M. D. (2019) Optimization of Metabolic Labeling with Alkyne-Containing Isoprenoid Probes, in Protein Lipidation: Methods and Protocols (Linder, M., Ed.), Springer, New York, p 35-44, https://link.springer.com/protocol/10.1007%2F978-1-4939-9532-5_3.
165. Suazo, K. F.; Schey, G.; Schaber, C.; John, A. R. O.; Distefano, M. D. (2019) Proteomic analysis of protein-lipid modifications: significance and application, in Mass Spectrometry-based Chemical Proteomics (Tao, W. A., Ed.), Wiley, New York, p 317-348, https://onlinelibrary.wiley.com/doi/pdf/10.1002/9781118970195.ch13.
164. Weber, D. K.; Bader, T.; Larsen, E. K.; Wang, S.; Gopinath, T.; Distefano, M. D.; Veglia, G. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy. Meth. Enzymol., 2019, 621, 281-304, doi.org/10.1016/bs.mie.2019.02.001.
163. Bader, T. K.; Rappe, T. M.; Veglia, G.; Distefano, M. D. Synthesis and NMR Characterization of the Prenylated Peptide, a-Factor. Meth. Enzymol., 2019, 614, 207-238, doi.org/10.1016/bs.mie.2018.09.025.
2018
162. Zhang, Y.; Park, K-. Y.; Suazo, K. F.; and Distefano, M. D. Recent progress in enzymatic protein labelling techniques and their applications. Chem. Soc. Rev., 2018, 47, 9106-9136, DOI: 10.1039/C8CS00537K
161. Suazo, K. F.; Hurben, A. K.; Liu, K.; Xu, F.; Thao, P., Sudheer, C.; Li, L.; Distefano, M. D. Metabolic Labeling of Prenylated Proteins Using Alkyne-Modified Isoprenoid Analogues. Curr. Protoc. Chem. Biol. 2018, 10, e46, PMC6158066, doi: 10.1002/cpch.46.
160. Jeong, A.; Suazo, K. F.; Wood, W. G.; Distefano, M. D. and Li, L. Isoprenoids and protein prenylation: implications in the pathogenesis and therapeutic intervention of Alzheimer’s disease. Crit. Rev. Biochem. Mol. Biol. 2018, 53, 279–310. doi.org/10.1080/10409238.2018.1458070
159. Pujari, S. S.; Zhang, Y.; Ji, S.; Distefano, M. D. and Tretyakova, N. T. Site-specific cross-linking of proteins to DNA via a new bioorthogonal approach employing oxime ligation. Chem. Commun., 2018, 54, 6296-6299, DOI: 10.1039/c8cc01300d
158. Fisher, S. A; Tam, R. T.; Fokina, A.; Mahmoodi, M. M.; Distefano, M. D.; Shoichet, M. S. Photo-Immobilized EGF Chemical Gradients Differentially Impact Breast Cancer Cell Invasion and Drug Response in Defined 3D Hydrogels. Biomaterials, 2018, 178, 751-766. doi.org/10.1016/j.biomaterials.2018.01.032
157. Blanden, M. J.; Suazo, K. F.; Hildebrandt, E. R.; Hardgrove, D. S.; Patel, M.; Saunders, W. P.; Distefano, M. D.; Schmidt, W. K.; Hougland, J. L., Efficient farnesylation of an extended C-terminal C(x)3X sequence motif expands the scope of the prenylated proteome. J. Biol. Chem. 2018, 293, 2770-2785, PMC5827447, doi: 10.1074/jbc.M117.805770
156. Diaz-Rodriguez, V., Hsu, E. T., Ganusova, E., Werst, E. R., Becker, J. M., Hrycyna, C. A., and Distefano, M. D., a-Factor Analogues Containing Alkyne- and Azide-Functionalized Isoprenoids Are Efficiently Enzymatically Processed and Retain Wild-Type Bioactivity. Bioconjug. Chem. 2018, 29, 316–323, PMC5824361, DOI: 10.1021/acs.bioconjchem.7b00648
2017
155. Diaz-Rodriguez, V.; Distefano, M. D., a-Factor: a chemical biology tool for the study of protein prenylation. Curr. Topics Pep. Prot. Res. 2017, 18, 133-151, PMC5915288.
154. Knutson, C. M.; Schneiderman, D. K.; Yu, M.; Javner, C.; Distefano, M. D. Wissinger, J. E. Polymeric Medical Sutures: An Exploration of Polymers and Green Chemistry. J. Chem. Ed. 2017, 94, 1761-1765, DOI: 10.1021/acs.jchemed.6b00835
2016
153. Suazo, K. F.; Schaber, C.; Palsuledesai, C. C.; Odom John, A. R.; Distefano, M. D., Global proteomic analysis of prenylated proteins in Plasmodium falciparum using an alkyne-modified isoprenoid analogue. Sci. Rep. 2016, 6, 38615. DOI: 10.1038/srep38615
152. Wickramaratne, S.; Ji, S.; Mukherjee, S.; Su, Y.; Pence, M. G.; Lior-Hoffmann, L.; Fu, I.; Broyde, S.; Guengerich, F. P.; Distefano, M.; Schärer, O. D.; Sham, Y. Y.; Tretyakova, N., Bypass of DNA-Protein Cross-links Conjugated to the 7-Deazaguanine Position of DNA by Translesion Synthesis Polymerases. J. Biol. Chem. 2016, 291, 23589-23603. DOI: 10.1074/jbc.M116.745257
151. Palsuledesai, C. C.; Ochocki, J. D.; Kuhns, M. M.; Wang, Y.-C.; Warmka, J. K.; Chernick, D. S.; Wattenberg, E. V.; Li, L.; Arriaga, E. A.; Distefano, M. D., Metabolic Labeling with an Alkyne-modified Isoprenoid Analog Facilitates Imaging and Quantification of the Prenylome in Cells. ACS Chem. Biol. 2016, 11, 2820-2828. DOI: 10.1021/acschembio.6b00421
150. Mahmoodi, M. M.; Fisher, S. A.; Tam, R. Y.; Goff, P. C.; Anderson, R. B.; Wissinger, J. E.; Blank, D. A.; Shoichet, M. S.; Distefano, M. D., 6-Bromo-7-hydroxy-3-methylcoumarin (mBhc) is an efficient multi-photon labile protecting group for thiol caging and three-dimensional chemical patterning. Org. Biomol. Chem. 2016, 14, 8289 - 8300. DOI: 10.1039/C6OB01045H
149. Mahmoodi, M. M.; Abate-Pella, D.; Pundsack, T. J.; Palsuledesai, C. C.; Goff, P. C.; Blank, D. A.; Distefano, M. D. Nitrodibenzofuran: A One- and Two-Photon Sensitive Protecting Group That Is Superior to Brominated Hydroxycoumarin for Thiol Caging in Peptides. J. Am. Chem. Soc. 2016 138, 5848-5859. DOI: 10.1021/jacs.5b11759
148. Jennings, B. C.; Danowitz, A. M.; Wang, Y.-C.; Gibbs, R. A.; Distefano, M. D.; Fierke, C. A., Analogs of farnesyl diphosphate alter CaaX substrate specificity and reactions rates of protein farnesyltransferase. Bioorg. Med. Chem. Let. 2016, 26, 1333-1336. doi.org/10.1016/j.bmcl.2015.12.079
147. Mohammed, I.; Hampton, S. E.; Ashall, L.; Hildebrandt, E. R.; Kutlik, R. A.; Manandhar, S. P.; Floyd, B. J.; Smith, H. E.; Dozier, J. K.; Distefano, M. D.; Schmidt, W. K.; Dore, T. M., 8-Hydroxyquinoline-based inhibitors of the Rce1 protease disrupt Ras membrane localization in human cells. Bioorg. Med. Chem. 2016, 24, 160-178. doi.org/10.1016/j.bmc.2015.11.043
146. Wang, Y.-C.; Distefano, M. D., Synthetic isoprenoid analogues for the study of prenylated proteins: Fluorescent imaging and proteomic applications. Bioorg. Chem. 2016, 64, 59-65. doi.org/10.1016/j.bioorg.2015.12.003
2015
145. Zhang, Y.; Blanden, M. J.; Sudheer, C.; Gangopadhyay, S. A.; Rashidian, M.; Hougland, J. L.; Distefano, M. D., Simultaneous Site-Specific Dual Protein Labeling Using Protein Prenyltransferases. Bioconj. Chem. 2015, 26, 2542-2553. DOI: 10.1021/acs.bioconjchem.5b00553
144. Diaz-Rodriguez, V.; Ganusova, E.; Rappe, T.M.; Becker, J.M.; Distefano, M.D. Synthesis of peptides containing c-terminal esters using trityl side-chain anchoring: Applications to the synthesis of C-terminal ester analogs of the Saccharomyces cerevisiae mating pheromone a-factor. J. Org. Chem. 2015, 80, 11266–11274. DOI: 10.1021/acs.joc.5b01376
143. Dozier, J.K.; Distefano, M.D. Site-specific pegylation of therapeutic proteins. Int. J. Mol. Sci. 2015, 16, 25831-35864. doi:10.3390/ijms161025831
142. Laudenbach, M.; Baruffaldi, F.; Vervacke, J.S.; Distefano, M.D.; Titcombe, P.J.; Mueller, D.L.; Tubo, N.J.; Griffith, T.S.; Pravetoni, M. The frequency of naive and early-activated hapten-specific b cell subsets dictates the efficacy of a therapeutic vaccine against prescription opioid abuse. J. Immunol. 2015, 194, 5926-5936. doi.org:10.4049:jimmunol.1500385
141. Mahmoodi, M. M.; Rashidian, M.; Zhang, Y.; Distefano, M. D. Application of meta- and para-Phenylenediamine as Enhanced Oxime Ligation Catalysts for Protein Labeling, PEGylation, Immobilization, and Release. Curr. Protoc. Protein Sci. 2015, 79, 15.4.1. DOI: 10.1002/0471140864.ps1504s79
140. Wickramaratne, S.; Boldry, E.; Buehler, C.; Wang, Y.-C.; Distefano, M. D.; Tretyakova, N. Y. Error-prone Translesion Synthesis Past DNA-Peptide Cross-links Conjugated to the Major Groove of DNA. J. Biol. Chem. 2015, 920, 775-787. doi: 10.1074/jbc.M114.613638
139. Palsuledesai, C. C.; Distefano, M. D. Protein prenylation: enzymes, therapeutics and biotechnology applications. ACS Chem. Biol. 2015, 10, 51-62. DOI: 10.1021/cb500791f
2014
138. Wang, Y.-C.; Distefano, M. D., Synthesis and screening of peptide libraries with free C-termini. Curr. Topics. Pep. Prot. Sci. 2014, 15, 1-23.
137. Dozier, J. K.; Khatwani, S. L.; Wollack, J. W.; Wang, Y.-C.; Schmidt-Dannert, C.; Distefano, M. D. Engineering Protein Farnesyltransferase for Enzymatic Protein Labeling Applications. Bioconj. Chem. 2014, 25, 1203-1212. DOI: 10.1021/bc500240p
136. Wang, Y.-C.; Dozier, J. K.; Beese, L. S.; Distefano, M. D. Rapid Analysis of Protein Farnesyltransferase Substrate Specificity Using Peptide Libraries and Isoprenoid Diphosphate Analogues. ACS Chem. Biol. 2014, 9, 1726-1735. DOI: 10.1021/cb5002312
135. Yeo, J. E.; Wickramaratne, S.; Khatwani, S.; Wang, Y.-C.; Vervacke, J.; Distefano, M. D.; Tretyakova, N. Y. Synthesis of Site-Specific DNA-Protein Conjugates and Their Effects on DNA Replication. ACS Chem. Biol. 2014, 9, 1860-1868. DOI: 10.1021/cb5001795
134. Pravetoni, M.; Vervacke, J. S.; Distefano, M. D.; Tucker, A. M.; Laudenbach, M.; Pentel, P. R. Effect of Currently Approved Carriers and Adjuvants on the Pre-Clinical Efficacy of a Conjugate Vaccine against Oxycodone in Mice and Rats. PLoS ONE 2014, 9, e96547. doi: 10.1371/journal.pone.0096547
133. Wollack, J. W.; Monson, B. J.; Dozier, J. K.; Dalluge, J. J.; Poss, K.; Hilderbrand, S. A.; Distefano, M. D. Site-Specific Labeling of Proteins and Peptides with Trans-cyclooctene Containing Handles Capable of Tetrazine Ligation. Chem. Biol. Drug Des. 2014, 84, 140-147. DOI: 10.1111/cbdd.12303
132. Palsuledesai, C. C.; Ochocki, J. D.; Markowski, T. W.; Distefano, M. D. A combination of metabolic labeling and 2D-DIGE analysis in response to a farnesyltransferase inhibitor facilitates the discovery of new prenylated proteins. Mol. Biosys. 2014, 10, 1094-1103. doi: 10.1039/c3mb70593e
131. Schuld, N. J.; Vervacke, J. S.; Lorimer, E. L.; Simon, N. C.; Hauser, A. D.; Barbieri, J. T.; Distefano, M. D.; Williams1, C. L. The chaperone protein SmgGDS interacts with small GTPases entering the prenylation pathway by recognizing the last amino acid in the CAAX motif. J. Biol. Chem. 2014, 289, 6862-6876. doi: 10.1074/jbc.M113.527192
130. Vervacke, J. S.; Funk, A. L.; Wang, Y.-C.; Strom, M.; Hrycyna, C. A.; Distefano, M. D. A Diazirine-Containing Photoactivatable Isoprenoid: Synthesis and Application in Studies with Isoprenylcysteine Carboxyl Methyltransferase. J. Org. Chem. 2014, 79, 1971-1978. DOI: 10.1021/jo402600b
2013
129. Tolstyka, Z. P.; Richardson, W.; Bat, E.; Stevens, C. J.; Parra, D. P.; Dozier, J. K.; Distefano, M. D.; Dunn, B.; Maynard, H. D. Chemoselective Immobilization of Proteins by Microcontact Printing and Bio-orthogonal Click Reactions. ChemBioChem 2013, 14, 2464-2471.
128. Rashidian, M.; Kumarapperuma, S. C.; Gabrielse, K.; Fegan, A.; Wagner, C. R.; Distefano, M. D. Simultaneous Dual Protein Labeling Using a Triorthogonal Reagent. J. Am. Chem. Soc. 2013, 135, 16388–16396.
127. Rashidian, M.; Dozier, J. K.; Distefano, M. D. Chemoenzymatic Labeling of Proteins: Techniques and Approaches. Bioconj. Chem. 2013, 24, 1277-1294.
126. Ochocki, J. D.; Igbavboa, U.; Wood, W. G.; Arriaga, E. A.; Wattenberg, E. V.; Distefano, M. D. Evaluation of prenylated peptides for use in cellular imaging and biochemical analysis. Meth. Mol. Biol. 2013, 1088, 213-223.
125. Ochocki, J. D.; Distefano, M. D. Prenyltransferase inhibitors: treating human ailments from cancer to parasitic infections. Med. Chem. Commun. 2013, 4, 476-492.
124. Mahmoodi, M. M.; Rashidian, M.; Dozier, J. K.; Distefano, M. D. Chemoenzymatic Site-Specific Reversible Immobilization and Labeling of Proteins from Crude Cellular Extract Without Prior Purification Using Oxime and Hydrazine Ligation. Curr. Protoc. Chem. Biol. 2013, 5, 89-109.
123. Wang, H.; Henry, O.; Distefano, M. D.; Wang, Y.-C.; Räikkönen, J.; Mönkkönen, J.; Tanaka, Y.; Morita, C. T. Butyrophilin 3A1 Plays an Essential Role in Prenyl Pyrophosphate Stimulation of Human Vγ2Vδ2 T Cells. J. Immunol. 2013, 191, 1029-1042.
122. Vervacke, J. S.; Wang, Y.-C.; Distefano, M. D. Photoactive analogues of farnesyl diphosphate and related isoprenoids: design and applications in studies of medicinally important isoprenoid-utilizing enzymes. Curr. Med. Chem. 2013, 20, 1585-1594.
121. Satori, C. P.; Henderson, M. M.; Krautkramer, E. A.; Kostal, V.; Distefano, M. D.; Arriaga, E. A. Bioanalysis of Eukaryotic Organelles. Chem. Rev. 2013, 113, 2733-2811.
120 Rashidian, M.; Mahmoodi, M. M.; Shah, R.; Dozier, J. K.; Wagner, C. R.; Distefano, M. D. A Highly Efficient Catalyst for Oxime Ligation and Hydrazone-Oxime Exchange Suitable for Bioconjugation. Bioconj. Chem. 2013, 24, 333-342.
119. Wang, Y.-C.; Distefano, M. D. "Solid-Phase Synthesis of C-Terminal Peptide Libraries for Studying the Specificity of Enzymatic Protein Farnesyltransferase" In Peptides across the Pacific: Proceedings of the 23rd American Peptide Symposium; Lebl, M., Ed.; Prompt Scientific Publishing: San Diego, 2013, p 102-103.
118. Mahmoodi, M.; Kubo, T.; Abate-Pella, D.; Wissinger, J. E.; Distefano, M. D. "Solvent-Free Synthesis of Bhc-Cl and Application to Cysteine Protection in Solid Phase Peptide Synthesis" In Peptides across the Pacific: Proceedings of the 23rd American Peptide Symposium; Lebl, M., Ed.; Prompt Scientific Publishing: San Diego, 2013, p 248-249.
117. Diaz-Rodriguez, V.; Ganusova, E.; Becker, J. M.; Distefano, M. D. "Synthesis and Bioactivity Analysis of a-Factor Analogs Containing Different Alkyl Esters at the C-Terminal Cysteine Using Trityl Side-Chain Anchoring" In Peptides across the Pacific: Proceedings of the 23rd American Peptide Symposium; Lebl, M., Ed.; Prompt Scientific Publishing: San Diego, 2013, p 206-207.
116. Vervacke, J.; Funk, A.; Schuld, N.; Williams, C.; Hrycnya, C.; Distefano, M. D. "Photoactive Prenylated Peptides for Studying Protein Isoprenylation" In Peptides across the Pacific: Proceedings of the 23rd American Peptide Symposium; Lebl, M., Ed.; Prompt Scientific Publishing: San Diego, 2013, p 208-209.
2012
115. Abate-Pella, D.; Distefano, M. D. In Cysteine: Biosynthesis, Chemical Structure and Toxicity; Chorkina, F. V., Karataev, A. I., Eds.; Nova Science Publishe: Hauppauge, NY, 2012.
114. Diaz-Rodriguez, V.; Mullen, D. G.; Ganusova, E.; Becker, J. M.; Distefano, M. D. Synthesis of Peptides Containing C-Terminal Methyl Esters Using Trityl Side-Chain Anchoring: Application to the Synthesis of a-Factor and a-Factor Analogs. Org. Lett. 2012, 14, 5648-5651. DOI: 10.1021/acs.joc.5b01376
113. Wang, Y.-C.; Distefano, M. D., Solid-Phase Synthesis of C-Terminal Peptide Libraries for Studying the Specificity of Enzymatic Protein Prenylation. Chem. Comm. 2012, 1359-7345.
112. Hahne, K.; Vervacke, J. S.; Shrestha, L.; Donelson, J. L.; Gibbs;, R. A.; Distefano, M. D.; Hrycyna, C. A., Evaluation of Substrate and Inhibitor Binding to Yeast and Human Isoprenylcysteine Carboxyl Methyltransferases (Icmts) using Biotinylated Benzophenone-containing Photoaffinity Probes. Biochem. Biophys. Res. Comm. 2012, 423, 98-103.
111. Khatwani, S. L.; Kang, J. S.; Mullen, D. G.; Hast, M. A.; Beese, L. S.; Distefano, M. D.; Taton, T. A., Covalent protein-oligonucleotide conjugates by copper-free click reaction. Bioorg. Med. Chem. 2012, 20, 4532-4539.
110. Rashidian, M.; Song, J. M.; Pricer, R. E.; Distefano, M. D., Chemoenzymatic Reversible Immobilization and Labeling of Proteins without Prior Purification. J. Am. Chem. Soc. 2012, 134, 8455–8467.
109. Dozier, J. K.; Distefano, M. D., An enzyme-coupled continuous fluorescence assay for farnesyl diphosphate synthases. Anal. Biochem. 2012, 421, 158-163.
108 Abate-Pella, D.; Zeliadt, N. A.; Ochocki, J. D.; Warmka, J. K.; Dore, T. M.; David A. Blank; Wattenberg, E. V.; Distefano, M. D., Photochemical Modulation of Ras-Mediated Signal Transduction using Caged Farnesyltransferase Inhibitors: Activation via One- and Two-Photon Excitation. ChemBioChem 2012, 13, 1009-1016.
2011
107. Kyro, K.; Manandhar, S. P.; Mullen, D.; Schmidt, W. K.; Distefano, M. D., Photoaffinity Labeling of Ras Converting Enzyme using Peptide Substrates that Incorporate Benzoylphenylalanine (Bpa) Residues: Improved Labeling and Structural Implications. Bioorg. Med. Chem. 2011, 19, 7559-7569.
106. Ochocki, J. D.; Mullen, D. G.; Wattenberg, E. V.; Distefano, M. D., Evaluation of a cell penetrating prenylated peptide lacking an intrinsic fluorophore via in situ click reaction. Bioorg. Med. Chem. Lett. 2011, 21, 4998-5001.
105. Gaffarogullari, E. C.; Masterson, L. R.; Metcalfe, E. E.; Traaseth, N. J.; Balatri, E.; Musa, M. M.; Mullen, D.; Distefano, M. D.; Veglia, G., A Myristoyl/Phosphoserine Switch Controls cAMP-Dependent Protein Kinase Association to Membranes. J. Mol. Bio. 2011, 411, 823-836.
104. Mullen, D.; Kyro, K.; Hauser, M.; Becker, J. M.; Naider, F.; Distefano, M. D., Synthesis of a-factor peptide from Saccharomyces cerevisiae and photoactive analogues via Fmoc solid phase methodology. Bioorg. Med. Chem. 2011, 19, 490-497.
103. Lenevich, S.; Distefano, M. D., NMR-based quantification of organic diphosphates. Anal. Biochem. 2011, 408, 316-320.
102. Wang, Y.-C.; Distefano, M. Preparation and Analysis of One-Bead One-Compound Libraries for Studying Protein Prenylation Specificity. In Building Bridges: Proceedings of the 22nd American Peptide Symposium; Lebl, M., Ed.; Prompt Scientific Publishing: San Diego, 2011, p 276-277.
101. Rashidian, M.; Distefano, M. D. Site-Specific Protein Labeling Via Oxime and Hydrazone Ligations Using Protein Farnesyltransferases. In Building Bridges: Proceedings of the 22nd American Peptide Symposium; Lebl, M., Ed.; Prompt Scientific Publishing: San Diego, 2011, p 88-89.
100. Kyro, K.; Schmidt, W. K.; Distefano, M. D. Design and Application of Bpa-Containing Peptides for Studying Ras-Converting Enzyme. In Building Bridges: Proceedings of the 22nd American Peptide Symposium; Lebl, M., Ed.; Prompt Scientific Publishing: San Diego, 2011, p 268-269.
99. Díaz-Rodríguez, V.; Mullen, D.; Distefano, M. D. Synthesis of a-Factor and a-Factor Analogs Using a Cysteine-Anchoring Method. In Building Bridges: Proceedings of the 22nd American Peptide Symposium; Lebl, M., Ed.; Prompt Scientific Publishing: San Diego, 2011, p 92-93.
98. Abate-Pella, D.; Distefano, M. D. Photocaged Cysteine Peptides for Studying Protein Farnesylation. In Building Bridges: Proceedings of the 22nd American Peptide Symposium; Lebl, M., Ed.; Prompt Scientific Publishing: San Diego, 2011, p 90-91.
2010
97. Rashidian, M.; Dozier, J. K.; Lenevich, S.; Distefano, M. D., Selective Labeling of Polypeptides Using Protein Farnesyltransferase via Rapid Oxime Ligation. Chem. Comm. 2010, 46, 8998-9000, DOI: 10.1039/C0CC03305G.
96. DeGraw, A. J.; Palsuledesai, C.; Ochocki, J. D.; Dozier, J. K.; Lenevich, S.; Rashidian, M.; Distefano, M. D., Evaluation of alkyne-modified isoprenoids as chemical reporters of protein prenylation. Chem. Bio. Drug. Des. 2010, 76, 460-471.
95. Kyro, K.; Manandhar, S.; Mullen, D.; Schmidt, W. K.; Distefano, M. D., Photoaffinity Labeling of Ras Converting Enzyme 1 (RCE1) using a Benzophenone-Containing Peptide Substrate. Bioorg. Med. Chem. 2010, 18, 5675-5684.
94. Ochocki, J. D.; Wattenberg, E. V.; Distefano, M. D., Enlarging the scope of cell penetrating prenylated peptides to include farnesylated “CAAX” box sequences and diverse cell types. Chem. Biol. Drug Des. 2010, 76, 107-115.
93. Lopez-Gallego, F.; Agger, S. A.; Abate-Pella, D.; Distefano, M. D.; Schmidt-Dannert, C., Sesquiterpene synthases Cop4 and Cop6 from Coprinus cinereus: catalytic promiscuity and cyclization of farnesyl pyrophosphate geometric isomers. Chembiochem. 2010, 11, 1093-1106.
92. Mullen, D.; Weigel, B.; Barany, G.; Distefano, M. D., On-resin conversion of Cys(Acm)-containing peptides to their corresponding Cys(Scm) congeners: scope and limitations. J. Peptide Sci. 2010, 16, 219-222.
91. DeGraw, A. J.; Keiser, M. J.; Ochocki, J. D.; Shoichet, B. K.; Distefano, M. D., Prediction and evaluation of protein farnesyltransferase inhibition by commercial drugs J. Med. Chem. 2010, 53, 2464–2471.
90. Wollack, J. W.; Zeliadt, N. A.; Mullen, D. G.; Wattenberg, E. V.; Barany, G.; Distefano, M. D., Investigation of the Sequence and Length Dependence for Cell-Penetrating Prenylated Peptides. Bioorg. Med. Chem. Lett. 2010, 20, 161-163.
89. Hovlid, M. L.; Edelstein, R. L.; Henry, O.; Ochocki, J.; DeGraw, A. J.; Talbot, T.; Lopez-Gallego, F.; Schmidt-Dannert, C.; Distefano, M. D., Synthesis, Properties and Applications of Diazotrifluropropanoyl-Containing Photoactive Analogues of Farnesyl Diphosphate Containing Modified Linkages for Enhanced Stability. Chem. Biol. Drug Des. 2010, 75, 51-67.
Prior to 2010
88. Wollack, J.; Silvermann, J.; Petzold, C.; Mougous, J.; Distefano, M. D., A Minimalist Substrate for Enzymatic Peptide and Protein Conjugation. ChemBiochem 2009, 10, 2934-2943.
87. Abate-Pella, D.; Zeliadt, N.; Mullen, D.; McDonald, P.; Pathak, P.; Dore, T.; Mueller, J.; Wattenberg, E.; Distefano, M. D., A Photolabile Thiol Protecting Group for Cellular Studies. In Breaking Away: Proceedings of the 21st American Peptide Symposium, Lebl, M., Ed. Prompt Scientific Publishing: San Diego, 2009; pp 362-363.
86. Wollack, J. W.; Katzenmeyer, J.; Ochocki, J. D.; Zeliadt, N. A.; Mullen, D. G.; Arriaga, E. A.; Wattenberg, E. V.; Distefano, M. D., Utilization of Hydrophobic Modification to Promote Internalization of a CAAX Box-containing Peptide. In Breaking Away: Proceedings of the 21st American Peptide Symposium, Lebl, M., Ed. Prompt Scientific Publishing: San Diego, 2009; pp 318-320.
85. Kyro, K.; Mullen, D.; Manandhar, S.; Walter K. Schmidt; Distefano, M. D., Photoactive Isoprenoid-containing Peptides. In Breaking Away: Proceedings of the 21st American Peptide Symposium, Lebl, M., Ed. Prompt Scientific Publishing: San Diego, 2009; pp 304-305.
84. Wollack, J. W.; Zeliadt, N. A.; Mullen, D. G.; Amundson, G.; Geier, S.; Falkum, S.; Wattenberg, E. V.; Barany, G.; Distefano, M. D., Multifunctional prenylated peptides for in vivo analysis. J. Am. Chem. Soc. 2009, 131, 7293–7303.
83. Henry, O.; Lopez-Gallego, F.; Agger, S. A.; Schmidt-Dannert, C.; Sen, S.; Shintani, D.; Cornish, K.; Distefano, M. D., A Versatile Photoactivatable Probe Designed to Label the Diphosphate Binding Site of Farnesyl Diphosphate Utilizing Enzymes. Bioorg. Med. Chem. 2009, 17, 4797-4805.
82. Chung, J. A.; Wollack, J. W.; Okesli, A.; Hovlid, M. L.; Chen, Y.; Mueller, J. D.; Distefano, M. D.; Taton, T. A., Purification of prenylated proteins by affinity chromatography on cyclodextrin-modified agarose. Anal. Biochem. 2009, 386, 1-8.
81. Sarikonda, G.; Wang, H.; Puan, K.-J.; Liu, X.-h.; Lee, H. K.; Song, Y.; Distefano, M. D.; Oldfield, E.; Prestwich, G. D.; Morita, C. T., Photoaffinity Antigens for Human gamma-delta T Cells. J. Immunol. 2008, 181, (11), 7738-7750.
80. DeGraw, A. J.; Hast, M. A.; Xu, J.; Mullen, D.; Beese, L. S.; Barany, G.; Distefano, M. D., Caged Protein Prenyltransferase Substrates: Tools for Understanding Protein Prenylation. Chem. Biol. Drug Des. 2008, 72, 171-181.
79. Xie, W.; McMahan, C. M.; Distefano, M. D.; DeGraw;, A. J.; Cornish, K.; Whalen, M. C.; Shintani, D. K., Initiation of rubber biosynthesis: in vitro comparisons of benzophenone-modified diphosphate analogues in three rubber-producing species. Phytochemistry, 2008, 69, 2539-2545.
78. Lenevich, S.; Hosokawa, A.; Cramer, C. J.; Distefano, M. D., Transition State Analysis of Model and Enzymatic Prenylation Reactions. J. Am. Chem. Soc. 2007, 129, 5796-5797.
77. Hosokawa, A.; Wollack, J. W.; Zhang, Z.; Chen, L.; Barany, G.; Distefano, M. D., Evaluation of an alkyne-containing analogue of farnesyl diphosphate as a dual substrate for protein-prenyltransferases. Int. J. Peptide Res. 2007, 13, (1-2), 345-354.
76. Duckworth, B. P.; Zhang, Z.; Hosokawa, A.; Distefano, M. D., Selective Labeling of Proteins using Protein Farnesyltransferase. ChemBioChem 2007, 8, (1), 98-105.
75. Duckworth, B. P.; Chen, Y.; Sham, Y.; Mueller, J. D.; Taton, T. A.; Distefano, M. D., A Universal Method for the Preparation of Covalent Protein-DNA Conjugates for Use in Creating Protein Nanostructures. Angewante Chemie (Int. Ed) 2007, 46, 8819-8822.
74. DeGraw, A. J.; Zhao, Z.; Hsieh, J.; Jefferies, M.; Distefano, M. D.; Strickland, C. L.; Shintani, D.; Nural, H.; McMahan, C.; Xie, W., A photoactive isoprenoid diphosphate analogue containing a stable phosphonate linkage: synthesis and structural biochemical studies with prenyltransferases. J. Org. Chem. 2007, 72, (13), 4587 - 4595.
73. Xu, J.; DeGraw, A. J.; Duckworth, B. P.; Lenevich, S.; Tann, C.-M.; Jenson, E. C.; Gruber, S. J.; Barany, G.; Distefano, M. D., Synthesis and Reactivity of 6,7-Dihydrogeranylazides: Reagents for Primary Azide Incorporation into Peptides and Subsequent Staudinger Ligation. Chem. Biol. Drug Des. 2006, 68, 85-96.
72. Duckworth, B. P.; Xu, J.; Taton, T. A.; Guo, A.; Distefano, M. D., Site-Specific, Covalent Attachment of Proteins to a Solid Surface. Bioconj. Chem. 2006, 17, (4), 967-974.
71. Distefano, M. D.; Albers, L. N.; Xu, J.-H., Protein Prenylation. In Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine, ed.; Ruckpaul, K.; Ganten, D., Springer-Verlag: Heidelberg, Germany, 2006; pp 349-354.
70. Duckworth, B.; Distefano, M. D., Protein-based artificial enzymes. In Artificial Enzymes, ed.; Breslow, R., Wiley-VCH Verlag: Weinheim, Germany, 2005; p 109-132.
69. Rose, M. W.; Rose, N. D.; Boggs, J.; Lenevich, S.; Xu, J.; Barany, G.; Distefano, M. D., Evaluation of geranylazide and farnesylazide diphosphate for incorporation of prenylazides into a CAAX box-containing peptide using protein farnesyltransferase. J. Peptide Res. 2005, 65, 529-537.
68. Rose, M. W.; Xu, J.; Kale, T. A.; O'Doherty, G.; Barany, G.; Distefano, M. D., Enzymatic incorporation of orthogonally reactive prenylazide groups into peptides via protein farnesyltransferase: implications for selective protein labeling. Biopolymers 2005, 80, (2-3), 164-171.
67. Adams, D. N.; Kao, E. Y.-C.; Hypolite, C. L.; Distefano, M. D.; Hu, W.-S.; Letourneau, P. C., Growth cones turn and migrate up an immobilized gradient of the laminin IKVAV peptide. J. Neurobiol. 2004, 62, (1), 134-147.
66. Franklin, M. C.; Kadkhodayan, S.; Ackerly, H.; Alexandru, D.; Distefano, M. D.; Elliott, L. O.; Flygare, J. A.; Mausisa, G.; Okawa, D. C.; Ong, D.; Vucic, D.; Deshayes, K.; Fairbrother, W. J., Structure and Function Analysis of Peptide Antagonists of Melanoma Inhibitor of Apoptosis (ML-IAP). Biochemistry 2003, 42, (27), 8223-8231.
65. Kale, T. A.; Distefano, M. D., Diazotrifluoropropionamido-Containing Prenylcysteines: Syntheses and Applications for Studying Isoprenoid-Protein Interactions. Org. Lett. 2003, 5, (5), 609-612.
64. Kale, T. A.; Hsieh, S.-A. J.; Rose, M. W.; Distefano, M. D., Use of synthetic isoprenoid analogues for understanding protein prenyltransferase mechanism and structure. Curr. Topics Med. Chem. 2003, 3, (10), 1043-1074.
63. Kale, T. A.; Raab, C.; Yu, N.; Aquino, E.; Dean, D. C.; Distefano, M. D., Synthesis of high specific activity 35S-labelled N-methanesulfonyl farnesylcysteine and a photoactive analog. J. Lab. Cmpds. Radiopharm. 2003, 46, (1), 29-54.
62. Turek-Etienne, T. C.; Strickland, C. L.; Distefano, M. D., Biochemical and Structural Studies with Prenyl Diphosphate Analogues Provide Insights into Isoprenoid Recognition by Protein Farnesyl Transferase. Biochemistry 2003, 42, (13), 3716-3724.
61. Distefano, M. D.; Zhong, A.; Cochran, A. G., Quantifying -Sheet stability by phage display. J. Mol. Bio. 2002, 322, (1), 179-188.
60. Häring, D.; Lees, M. R.; Banaszak, L. J.; Distefano, M. D., Exploring routes to stabilize a cationic pyridoxamine in an artificial transaminase: site-directed mutagenesis versus synthetic cofactors. Protein Eng. 2002, 15, (7), 603-10.
59. Kale, T. A.; Turek, T. C.; Chang, V.; Gautam, N.; Distefano, M. D., Preparation and application of G protein g subunit-derived peptides incorporating a photoactive isoprenoid. Meth. Enzymol. 2002, 344, (G Protein Pathways, Part B), 245-258.
58. Clausen, V. A.; Edelstein, R. L.; Distefano, M. D., Stereochemical Analysis of the Reaction Catalyzed by Human Protein Geranylgeranyl Transferase. Biochemistry 2001, 40, (13), 3920-3930.
57. Häring, D.; Distefano, M. D., Enzymes by Design: Chemogenetic Assembly of Transamination Active Sites Containing Lysine Residues for Covalent Catalysis. Bioconj. Chem. 2001, 12, (3), 385-390.
56. Häring, D.; Distefano, M. D., Specific Host-Guest Interactions in a Protein-Based Artificial Transaminase. Bioorg. Med. Chem. 2001, 9, 2461-2466.
55. Häring, D.; Distefano, M. D., Converting a Fatty Acid Binding Protein to a Artificial Transaminase: Novel Catalysts by Chemical and Genetic Modification of a Protein Cavity. J. Mol. Catal. B, Enz. 2001, 11, 967-970.
54. Kale, T. A.; Raab, C.; Yu, N.; Dean, D. C.; Distefano, M. D., A Photoactivatable Prenylated Cysteine Designed to Study Isoprenoid Recognition. J. Am. Chem. Soc. 2001, 123, (19), 4373-4381.
53. Qi, D.; Tann, C.-M.; Häring, D.; Distefano, M. D., Generation of new enzymes via covalent modification of existing proteins. Chemical Reviews 2001, 101, (10), 3081-3111.
52. Tann, C.-M.; Qi, D.; Distefano, M. D., Enzyme design by chemical modification of protein scaffolds. Curr. Opin. Cell Biol. 2001, 5, (6), 696-704.
51. Turek, T. C.; Gaon, I.; Distefano, M. D.; Strickland, C. L., Synthesis of Farnesyl Diphosphate Analogues Containing Ether-Linked Photoactive Benzophenones and Their Application in Studies of Protein Prenyltransferases. J. Org. Chem. 2001, 66, (10), 3253-3264.
50. Distefano, M. D.; Kuang, H.; Qi, D.; Häring, D.; Ory, J.; Banaszak, L. J., Semisynthetic approaches for the design of proteins with catalytic activity using fatty acid binding protein as a scaffold. Peptides for the New Millennium, Proceedings of the American Peptide Symposium, 16th, Minneapolis, MN, United States, June 26-July 1, 1999 2000, 500-502.
49. Kale, T. A.; Distefano, M. D., Photoaffinity-labeled probes for the study of isoprenoid recognition sites. Peptides for the New Millennium, Proceedings of the American Peptide Symposium, 16th, Minneapolis, MN, United States, June 26-July 1, 1999 2000, 109-110.
48. Kuang, H.; Häring, D.; Qi, D.; Mazhary, A.; Distefano, M. D., Synthesis of a cationic pyridoxamine conjugation reagent and application to the mechanistic analysis of an artificial transaminase. Bioorg. Med. Chem. Lett. 2000, 10, 2091-2095.
47. Davies, R. R.; Kuang, H.; Qi, D.; Mazhary, A.; Mayaan, E.; Distefano, M. D., Artificial metalloenzymes based on protein cavities: exploring the effect of altering the metal ligand attachment position by site directed mutagenesis. Bioorg. Med. Chem. Lett. 1999, 9, (1), 79-84.
46. Distefano, M. D.; Kuang, H.; Qi, D.; Mazhary, A., The design of protein-based catalysts using semisynthetic methods. Curr. Opin. Struc. Biol. 1998, 8, (4), 459-465.
45. Edelstein, R. L.; Weller, V. A.; Distefano, M. D.; Tung, J. S., Stereochemical Analysis of the Reaction Catalyzed by Yeast Protein Farnesyltransferase. J. Org. Chem. 1998, 63, (16), 5298-5299.
44. Kuang, H.; Distefano, M. D., Catalytic Enantioselective Reductive Amination in a Host-Guest System Based on a Protein Cavity. J. Am. Chem. Soc. 1998, 120, (5), 1072-1073.
43. Ory, J. J.; Mazhary, A.; Kuang, H.; Davies, R. R.; Distefano, M. D.; Banaszak, L. J., Structural characterization of two synthetic catalysts based on adipocyte lipid-binding protein. Protein Eng. 1998, 11, (4), 253-261.
42. Qi, D.; Kuang, H.; Distefano, M. D., Effects of metal ions on the rates and enantioselectivities of reactions catalyzed by a series of semisynthetic transaminases created by site directed mutagenesis. Bioorg. Med. Chem. Lett. 1998, 8, (7), 875-880.
41. Tabakovic, I.; Gaon, I.; Distefano, M. D., Anodic oxidation of substituted [4-hydroxy-3-coumarinyl]-phenylthiomethanes in acetonitrile. Evidence for a cationic intermediate in carbon-sulfur bond fragmentation. Electrochim. Acta 1998, 43, (12-13), 1773-1778.
40. Turek, T. C.; Edelstein, R. L.; Gaon, I.; Weller, V. A.; Distefano, M. D., [32P]-labeled analogs of farnesyl and geranylgeranyl pyrophosphate: Synthesis and application in photoaffinity labeling experiments with protein prenyltransferases. In Synthesis and Applications of Isotopically Labelled Compounds 1997, Proceedings of the International Symposium, 6th, Philadelphia, PA, United States, Sept. 14-18, 1997, 1998; p 67-70.
39. Weller, V. A.; Distefano, M. D., Measurement of the -Secondary Kinetic Isotope Effect for a Prenyltransferase by MALDI Mass Spectrometry. J. Am. Chem. Soc. 1998, 120, (31), 7975-7976.
38. Kuang, H.; Distefano, M. D., Artificial enzymes based on protein cavities: inversion of reaction enantioselectivity by alteration of the site of catalyst attachment. Protein Eng. 1997, 10, 25.
37. Davies, R. R.; Brown, M. L.; Distefano, M. D., An artificial metalloenzyme based on a protein cavity that catalyzes the enantio-selective hydrolysis of amino acid esters. Protein Eng. 1997, 10, 61.
36. Hypolite, C. L.; McLernon, T.; Adams, D.; Herbert, C.; Huang, C. C.; Hu, W.-S.; Distefano, M. D., Two-dimensional surface gradients of photoactivable R-phycoerythrin. Protein Eng. 1997, 10, 84.
35. Davies, R. R.; Distefano, M. D., A Semisynthetic Metalloenzyme Based on a Protein Cavity That Catalyzes the Enantioselective Hydrolysis of Ester and Amide Substrates. J. Am. Chem. Soc. 1997, 119, (48), 11643-11652.
34. Edelstein, R. L.; Distefano, M. D., Photoaffinity Labeling of Yeast Farnesyl Protein Transferase and Enzymatic Synthesis of a Ras Protein Incorporating a Photoactive Isoprenoid. Biochem. Biophys. Res. Comm. 1997, 235, 377-382.
33. Herbert, C. B.; McLernon, T. L.; Hypolite, C. L.; Adams, D. N.; Pikus, L.; Huang, C. C.; Fields, G. B.; Letourneau, P. C.; Distefano, M. D.; Hu, W.-S., Micropatterning gradients and controlling surface densities of photoactivatable biomolecules on self-assembled monolayers of oligo(ethylene glycol) alkanethiolates. Chem. Biol. 1997, 4, (10), 731-737.
32. Hypolite, C. L.; McLernon, T. L.; Adams, D. N.; Chapman, K. E.; Herbert, C. B.; Huang, C. C.; Distefano, M. D.; Hu, W.-S., Formation of Microscale Gradients of Protein Using Heterobifunctional Photolinkers. Bioconj. Chem. 1997, 8, (5), 658-663.
31. Kuang, H.; Davies, R. R.; Distefano, M. D., Modulation of the rate, enantioselectivity, and substrate specificity of semisynthetic transaminases based on lipid binding proteins using site directed mutagenesis. Bioorg. Med. Chem. Lett. 1997, 7, (15), 2055-2060.
30. Turek, T. C.; Gaon, I.; Distefano, M. D., Synthesis and rapid purification of 32P-labeled photoactive analogs of farnesyl pyrophosphate. J. Labelled Comp. Radiopharm. 1997, 39, (2), 139-146.
29. Turek, T. C.; Gaon, I.; Gamache, D.; Distefano, M. D., Synthesis and evaluation of benzophenone-based photoaffinity labeling analogs of prenyl pyrophosphates containing stable amide linkages. Bioorg. Med. Chem. Lett. 1997, 7, (16), 2125-2130.
28. Gaon, I.; Turek, T. C.; Distefano, M. D., Farnesyl and geranylgeranyl pyrophosphate analogs incorporating benzoylbenzyl ethers: synthesis and inhibition of yeast protein farnesyltransferase. Tet. Lett. 1996, 37, (49), 8833-8836.
27. Edelstein, R. L.; Distefano, M. D., Photoaffinity Labeling using Photoreactive Isoprenoid Analogs. FASEB J. 1997, 11, A1087.
26. Weller, V. A.; Turek, T. C.; Gaon, I.; Distefano, M. D., Mechanistic and Structural Studies on Protein Prenyl Transferases. FASEB J. 1997, 11, A1086.
25. Ory, J. J.; Kuang, H.; Davies, R. R.; Mazhary, A.; Distefano, M. D.; Banaszak, L. J., Structural Characterization of Two Artificial Enzymes Based on Adipocyte Lipid Binding Protein. FASEB. J. 1997, 11, A1323.
24. Gaon, I.; Turek, T. C.; Weller, V. A.; Edelstein, R. L.; Singh, S. K.; Distefano, M. D., Photoactive Analogs of Farnesyl Pyrophosphate Containing Benzoylbenzoate Esters: Synthesis and Application to Photoaffinity Labeling of Yeast Protein Farnesyltransferase. J. Org. Chem. 1996, 61, (22), 7738-7745.
23. Kuang, H.; Brown, M. L.; Davies, R. R.; Young, E. C.; Distefano, M. D., Enantioselective Reductive Amination of -Keto Acids to -Amino Acids by a Pyridoxamine Cofactor in a Protein Cavity. J. Am. Chem. Soc. 1996, 118, (44), 10702-10706.
22. Turek, T. C.; Gaon, I.; Distefano, M. D., Analogs of farnesyl pyrophosphate incorporating internal benzoylbenzoate esters: synthesis, inhibition kinetics and photoinactivation of yeast protein farnesyltransferase. Tet. Lett. 1996, 37, (28), 4845-4848.
Postdoctoral Research
21. Colocci, N.; Distefano, M. D.; Dervan, P. B., Cooperative oligonucleotide-directed triple helix formation at adjacent DNA sites. J. Am. Chem. Soc. 1993, 115, (11), 4468-73.
20. Distefano, M. D.; Dervan, P. B., Energetics of cooperative binding of oligonucleotides with discrete dimerization domains to DNA by triple helix formation. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, (4), 1179-83.
19. Distefano, M. D.; Dervan, P. B., Ligand-promoted dimerization of oligonucleotides binding cooperatively to DNA. J. Am. Chem. Soc. 1992, 114, (27), 11006-7.
18. Distefano, M. D.; Shin, J. A.; Dervan, P. B., Cooperative binding of oligonucleotides to DNA by triple helix formation: dimerization via Watson-Crick hydrogen bonds. J. Am. Chem. Soc. 1991, 113, (15), 5901-2.
Graduate Research
17. Schiering, N.; Kabsch, W.; Moore, M. J.; Distefano, M. D.; Walsh, C. T.; Pai, E. F., Structure of the Detoxification Catalyst Mercuric Ion Reductase from Bacillus sp. strain RC607. Nature 1991, 352, 168-172.
16. Schiering, N.; Kabsch, W.; Moore, M. J.; Distefano, M. D.; Walsh, C. T.; Pai, E. F., The Three Dimensional Structure of Mercuric Ion Reductase from Bacillus Strain RC607. In Flavins and Flavoproteins 1990, ed.; Curti, B.; Ronchi, S.; Zanetti, G., Walter de Gruyter & Co.: Berlin, 1991; p 615-625.
15. Distefano, M. D.; Moore, M. J.; Walsh, C. T., Active site of mercuric reductase resides at the subunit interface and requires Cys135 and Cys140 from one subunit and Cys558 and Cys559 from the adjacent subunit: evidence from in vivo and in vitro heterodimer formation. Biochemistry 1990, 29, (11), 2703-13.
14. Miller, S. M.; Massey, V.; Ballou, D.; Williams, C. H., Jr.; Distefano, M. D.; Moore, M. J.; Walsh, C. T., Use of a site-directed triple mutant to trap intermediates: demonstration that the flavin C(4a)-thiol adduct and reduced flavin are kinetically competent intermediates in mercuric ion reductase. Biochemistry 1990, 29, (11), 2831-41.
13. Moore, M. J.; Distefano, M. D.; Zydowsky, L. D.; Cummings, R. T.; Walsh, C. T., Organomercurial lyase and mercuric ion reductase: nature's mercury detoxification catalysts. Acc. Chem. Res. 1990, 23, (9), 301-8.
12. Raybuck, S. A.; Distefano, M. D.; Teo, B. K.; Orme-Johnson, W.; Walsh, C. T., An EXAFS investigation of Hg(II) binding to mercuric reductase: comparative analysis of the wild-type enzyme and a mutant enzyme generated by site-directed mutagenesis. J. Am. Chem. Soc. 1990, 112, (5), 1983-9.
11. Distefano, M. D.; Au, K. G.; Walsh, C. T., Mutagenesis of the redox-active disulfide in mercuric ion reductase: catalysis by mutant enzymes restricted to flavin redox chemistry. Biochemistry 1989, 28, (3), 1168-83.
10. Miller, S. M.; Moore, M. J.; Massey, V.; Williams, C. H., Jr.; Distefano, M. D.; Ballou, D. P.; Walsh, C. T., Evidence for the participation of Cys558 and Cys559 at the active site of mercuric reductase. Biochemistry 1989, 28, (3), 1194-205.
9. Moore, M. J.; Distefano, M. D.; Walsh, C. T.; Schiering, N.; Pai, E. F., Purification, crystallization, and preliminary x-ray diffraction studies of the flavoenzyme mercuric ion reductase from Bacillus sp. strain RC607. J. Biol. Chem. 1989, 264, (24), 14386-8.
8. Walsh, C.; Distefano, M. D.; Moore, M., Mutagenesis of paired cysteine residues in the disulphide-containing flavoprotein mercuric ion reductase from mercury-resistant bacteria. Biochem. Soc. Trans. 1988, 16, (2), 90-1.
7. Walsh, C. T.; Distefano, M. D.; Moore, M. J.; Shewchuk, L. M.; Verdine, G. L., Molecular basis of bacterial resistance to organomercurial and inorganic mercuric salts. FASEB J. 1988, 2, (2), 124-30.
6. Massey, V.; Miller, S.; Ballou, D. P.; Williams, C. H.; Moore, M. J.; Distefano, M. D.; Walsh, C. T., Studies of the Active Site of Mercuric Reductase Employing Site Directed Mutants and the Thiol-Reactive Flavin, 6-SCN-FAD. In Flavins and Flavoproteins 1987, ed.; Edmondson, D. E.; McCormick, D. B., Walter de Gruyter & Co.: Berlin, 1987; p 41-44.
5. Moore, M. J.; Distefano, M. D.; Walsh, C. T.; Miller, S.; Massey, V.; Williams, C. H.; Ballou, D. P., The Penultimate Cysteines in Mercuric Reductase Aid in the Reduction of Mercury. In Flavins and Flavoproteins 1987, ed.; Edmondson, D. E.; McCormick, D. B., Walter de Gruyter & Co.: Berlin, 1987; p 37-40.
4. Neidhard, D. J.; Distefano, M. D.; Tanizawa, K.; Soda, K.; Walsh, C. T.; Petsko, G. A., X-ray crystallographic studies of the alanine-specific racemase from Bacillus stearothermophilus. Overproduction, crystallization, and preliminary characterization. J. Biol. Chem. 1987, 262, (32), 15323-6.
3. Walsh, C.; Distefano, M. D.; Moore, M., Catalytic effects of mutagenesis of paired cysteine residues in the bacterial enzyme mercuric ion reductase. UCLA Symposia on Molecular and Cellular Biology, New Series 1987, 69, (Protein Struct., Folding, Des. 2), p 283-92.
2. Walsh, C. T.; Moore, M. J.; Distefano, M. D., Conserved Cysteine Pairs of Mercuric Ion Reductase: An Investigation of Function Via Site-Directed Mutagenesis. In Flavins and Flavoproteins 1987, ed.; Edmondson, D. E.; McCormick, D. B.,Walter de Gruyter & Co.: Berlin, 1987; p 13-28.
Undergraduate Research
1. Kanazawa, T.; Distefano, M. D.; Bassham, J. A., Ammonia regulation of intermediary metabolism in photosynthesizing and respiring Chlorella pyrenoidosa: comparative effects of methylamine. Plant Cell Physiol. 1983, 24, (6), 979-86.